BcMag™ Cleavable Iodoacetyl-activated Magnetic Beads are uniform, silica-based superparamagnetic beads coated with a high density of cleavable iodoacetyl functional groups on the surface. It is designed to enable fast, efficient, and covalent immobilization of protein, peptides, and other ligands through their sulfhydryl groups (-SH) for affinity purification procedures. At physiological to alkaline circumstances (pH 7.2 to 9) in either aqueous or organic solvents 20- 30% DMSO or DMF, iodoacetyl-activated supports react with sulfhydryl groups, resulting in stable thioether bonds. These reactions are often carried out in the dark to prevent the formation of free iodine, which can react with tyrosine, histidine, and tryptophan residues. Since the active iodoacetyl group is linked with the beads through a built-in cleavable disulfide linker, reducing agents such as DTT or β-mercaptoethanol can cleave and separate the target molecule-ligand complex from the beads, and only a small sulfhydryl group is attached to ligand after affinity purification. Moreover, the hydrophilic surface ensures low nonspecific adsorption, excellent dispersion, and easy handling in various buffers. The beads are suitable for conjugating larger protein or small biomolecules without steric hindrance.

The iodoacetyl beads work perfectly as affinity resin for affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a sample containing the target molecules, then mix, incubate, wash and elute the target molecules.

Features and Benefits

●

Iodoacetyl groups react selectively with sulfhydryl (-SH) groups to produce irreversible thioether linkages.

●

Fast – couple peptide samples in 2 hours.

●

A cleavable built-in disulfide bond allows the ligand-target molecule complex to separate from the beads.

●

Versatile coupling conditions—as needed for protein or peptide solubility during the coupling reaction, employ pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO), or denaturant (guanidine HCl).

●

Simple to follow protocols for sample preparation, immobilization, and affinity purification

●

High capacity – Immobilize 15 -20 μg antibody/mg beads.

Applications

●

Immobilize peptides with terminal cysteine residues to purify antibodies raised against peptide immunogens.

●

Immobilize antibodies in an orientated manner using hinge-region sulfhydryls to ensure that antigen binding sites are not sterically inhibited when antigen affinity purification is performed.

●

Produces reusable immunoaffinity matrices.

●

Maintains antibody function—immobilizes IgG via the Fc region, leaving both antigen binding sites available for target capture.

Learn More

Instruction Manual

MSDS