BcMag™ Cleavable Aldehyde-Activated Magnetic Beads are uniform, silica-based superparamagnetic beads grafted with a high density of cleavable aldehyde functional groups on the surface. The bead aldehyde groups react spontaneously with primary amines present at the N-terminus of proteins or in lysine residues to form intermediate Schiff Base complexes. The reaction of reductive amination immobilization starts with the creation of an initial Schiff base between the aldehyde and amine groups, which is then reduced to a secondary amine by the addition of sodium cyanoborohydride (NaCNBH3) to generate stable amine bonds between the Bead and the ligand. At physiological to alkaline circumstances (pH 7.2 to 9) in either aqueous or organic solvents with 20- 30% DMSO or DMF, coupling reaction takes 2 to 6 hours in a one-step process. Coupling efficiency with antibodies and normal proteins is usually better than 85%, resulting in 15 – 20 µg /mg of beads.

The Cleavable Aldehyde-Activated Magnetic Beads are ideal for conjugating either larger protein or small peptides. Because the active aldehyde group is linked to the beads via a cleavable disulfide linker, reducing agents like DTT or -mercaptoethanol can cleave and separate the target molecule-ligand complex from the beads, leaving only a small sulfhydryl group attached to the ligand after affinity purification. Moreover, the hydrophilic surface ensures low nonspecific adsorption, excellent dispersion, and easy handling in various buffers.

The Aldehyde-Activated resins have higher coupling efficiency than cyanogen bromide (CNBr) activated supports. Furthermore, the aldehyde Chemistry generates an uncharged connection with the amine-containing ligand that is more stable than the CNBr approach. The hydrophilic surface ensures excellent dispersion and easy handling in various buffers. When utilized for affinity purification methods, these features allow better leak-resistant immobilization and lower nonspecific binding.

Workflow

The beads work perfectly as affinity resin for affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a sample containing the target molecules, then mix, incubate, wash and elute the target molecules.

Features and Advantages

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Pre-activated and ready-to-use

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A cleavable built-in disulfide bond allows the ligand-target molecule complex to separate from the beads.

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Quick, Easy, and one-step high-throughput procedure; eliminates columns or filters or a laborious repeat of pipetting or centrifugation.

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Stable covalent bond with minimal ligand leakage

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High capacity – Immobilize 15 -20 µg antibody/mg beads.

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Scalable – easily adjusts for sample size and automation

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Low nonspecific binding

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Reproducible results

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Application: Purification for antibody, protein/peptide, DNA/RNA, cell sorting, immunoprecipitation

Learn More

Instruction Manual

MSDS

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