BcMag™ Cleavable Carboxyl-Terminated Magnetic Beads are uniform, silica-based magnetic beads coated with a high density of carboxylic acid groups on the surface. The high density of carboxylic acid groups on their surface allows efficiently covalently conjugate primary amine-containing ligands via a stable amide bond. Since the active carboxyl group is linked with the beads through a built-in cleavable disulfide linker (Fig.1), reducing agents such as DTT or β-mercaptoethanol can cleave and separate the target molecule-ligand complex from the beads. The cleavable carboxyl magnetic beads are ideal matrices for conjugating large proteins or small peptides. Moreover, the hydrophilic surface ensures low nonspecific adsorption, excellent dispersion, and easy handling in various buffers.

The unique dry form eliminates the need for solvent storage or removal and disposal. Furthermore, because the dry resin concentrates the sample as it swells, lowering the volume of the starting material and resulting in highly effective ligand immobilization, it is perfect for coupling reactions with dilute materials.

Workflow

The Beads work perfectly as affinity resin for affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a sample containing the target molecules, then mix, incubate, wash and elute the target molecules.

Features and Advantages

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Easy to use

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A cleavable built-in disulfide bond allows the ligand-target molecule complex separated from the beads.

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Stable covalent bond with minimal ligand leakage

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Produces reusable immunoaffinity matrix

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Low nonspecific binding

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Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads

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Applications: Purification for antibodies, proteins/peptides, DNA/RNA, cell sorting,immunoprecipitation.

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Instruction Manual

MSDS