BcMag™ Cleavable Amine-Terminated Magnetic Beads are magnetic beads coated with a high density of primary amine functional groups on the surface. The beads can covalently conjugate primary amine or carboxy-containing ligands such as protein and peptides for affinity purification. Since the active amine group is linked with the beads through a built-in cleavable disulfide linker (Fig.1), reducing agents such as DTT or β-mercaptoethanol can cleave and separate the target molecule-ligand complex from the beads. Moreover, the hydrophilic surface ensures beads low nonspecific adsorption, excellent dispersion, and easy handling in various buffers. The beads are suitable for the conjugation of large-size proteins or small peptides.

Workflow

The amine beads work perfectly as affinity resin for affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a sample containing the target molecules, then mix, incubate, wash and elute the target molecules.

Features and Benefits

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Covalently coupled with high efficiency

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Easy to use

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Stable covalent bond with low levels of ligand leakage

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Produces reusable immunoaffinity matrix

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Low nonspecific binding

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Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads

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A cleavable built-in disulfide bond allows the ligand-target molecule complex to separate from the beads.

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Application: Purification for antibody, protein/peptide, DNA/RNA; cell sorting, immunoprecipitation

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Instruction Manual

MSDS