BcMag™ IDA Magnetic Beads are 1μm and 5μm in diameter and highly uniform magnetic microspheres covalently immobilized with a high density of IDA (Iminodiacetic Acid) ligand designed for capture and purification of proteins from various sample types. The microspheres combine all the advantages of Immobilized metal ion affinity chromatography (IMAC) protein purification such phosphoproteins, phosphopeptides or his-tagged protein (low costs, simplicity, high specificity, and capacity) and magnetic properties to perform efficient manual or automatic quick high-throughput purification.
Workflow
BcMag™ IDA Magnetic Beads work perfectly as solid resin for various affinity purifications to refine molecules, cells, and parts of cells into purified fractions. Add the beads to a solution containing the target molecules, then mix, incubate, wash, and elute the target molecules
Features and Advantages
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Easy to use
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More efficient and low nonspecific binding
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Stable covalent bond with minimal ligand leakage
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Produces reusable matrices
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Low nonspecific binding
Applications
To purify phosphoproteins and phosphopeptides, you can easily replace your current Al3+, Ga3+, or Fe3+ chelated resin or cartridge without needing to change your protocol or optimize the process. This method works for purification under both native and denaturing conditions and is effective for proteins of various sizes and with low expression rates.Phosphopeptides are often found in complex proteolytic digest mixtures containing both phosphorylated and nonphosphorylated components, and immobilized Al3+, Ga3+, and Fe3+ ions are commonly used to selectively enrich them. NTA and IDA are popular ligands used in this process, and different ligand-metal ion combinations can yield varying purification results depending on the protein’s identity, impurity profile, and purification conditions.How to choose different ligands in IMAC?
When selecting ligands for IMAC, NTA and IDA are the two most used options. NTA has a coordination number of 4 and is a tetravalent ligand, resulting in strong coordination of metal ions. In contrast, IDA has a coordination number of 3 and is a trivalent ligand. NTA typically provides higher binding specificity and lower metal ion leaching, while IDA has more non-specific binding and greater metal ion leaching. IDA can handle higher metal ion loading density and often requires a lower imidazole concentration in the eluent.
Learn More
Instruction Manual
MSDS
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