BcMagTM Tosyl Activated Magnetic Beads are magnetic beads grafted with tosyl functional groups on the surface. Short and long-arm tosyl-activated magnetic beads can efficiently conjugate ligands containing primary amine in either aqueous or organic solvents (30% DMF) without introducing any charge (Fig.1). The Tosyl Activated Magnetic Beads are suitable for conjugation of large size protein, while long-arm tosyl activated magnetic beads are ideal for coupling small molecules without steric hindrance problem.

The Tosyl Activated magnetic resins are an ideal choice for covalently attaching antibodies, peptides, complete proteins, and functional enzymes to the surface. The immobilized beads are widely used in Immunoprecipitation of proteins and protein complexes due to their low background and covalent binding of antibodies to the bead surface.

The Tosyl Activated magnetic resins coupling reaction is carried out at 37°C, and pH ranges from neutral to high. We advocate coupling at pH 8.5-9.5, but coupling with pH labile ligands can be done in an alternate buffer at pH 7.4.

The unique dry form eliminates the need for acetone solvent storage or removal and disposal. Furthermore, because the dry resin concentrates the sample as it swells, lowering the volume of the starting material and resulting in highly effective ligand immobilization, it is perfect for coupling reactions with dilute materials.

Workflow

The tosyl-activated magnetic beads work perfectly as solid support for various bioseparations to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a solution containing the target molecules, then mix, incubate, wash and elute the target molecules.

Features and Advantages

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Pre-activated and ready-to-use

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Easy to use

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No charge remains on the surface after coupling

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Stable covalent bond with minimal ligand leakage

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Produces reusable immunoaffinity matrices

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Low nonspecific binding

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Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads

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Applications: Cell sorting, Immunoprecipitation, purification for antibodies, proteins/peptides, DNA/RNA

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Instruction Manual

MSDS