BcMag™ Epoxy-Activated Magnetic Beads are pre-activated, uniform magnetic beads coated with high-density epoxy functional groups on the surface. The beads can covalently conjugate amine, sulfhydryl, or hydroxyl group-containing ligands (Fig.1). Moreover, the hydrophilic surface ensures beads have low nonspecific adsorption, excellent dispersion, and easy handling in various buffers. The epoxy-activated magnetic beads are most suitable for the conjugation of large proteins. BcMag™ Long-arm Epoxy-Activated Magnetic Beads are recommended to conjugate small peptides because the long-arm hydrophilic linker may reduce steric hindrance.

Any ligands such as antibodies, peptides, complete proteins, and functional enzymes can be covalently linked to the bead’s surface in a simple overnight reaction. Incubate the ligand you intend to use for affinity purification with these epoxy-activated beads at high pH (8.5-9.5) and 37°C. Coupling can be done in an alternate buffer at pH 7.4 for pH labile ligands.

The unique dry form eliminates the need for acetone solvent storage or removal and disposal. Furthermore, because the dry resin concentrates the sample as it swells, lowering the volume of the starting material and resulting in highly effective ligand immobilization, it is perfect for coupling reactions with dilute materials.

Workflow

BcMag™ Epoxy-Activated Magnetic Beads work perfectly as affinity resin for affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a sample containing the target molecules, then mix, incubate, wash and elute the target molecules.

Features and Advantages

●

Pre-activated and ready-to-use

●

Stable covalent bond with minimal ligand leakage

●

Produces reusable immunoaffinity matrices

●

Low nonspecific binding

●

Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads

●

Applications: Cell sorting, immunoprecipitation, purification for antibodies, proteins/peptides, DNA/RNA

Learn More

Instruction Manual

MSDS