BcMag™ Carboxyl-Terminated Magnetic Beads are uniform, silica-based magnetic beads coated with a high density of carboxylic acid groups on the surface. The hydrophilic surface ensures low nonspecific adsorption, excellent dispersion, and easy handling in various buffers. The high density of pendent functional carboxylic acid groups on their surface allows covalently conjugate primary amine-containing ligands via a stable amide bond. Moreover, the hydrophilic surface ensures beads low nonspecific adsorption, excellent dispersion, and easy handling in various buffers. BcMag™ Carboxyl-Terminated Magnetic Beads are most suitable for conjugating a larger protein. At the same time, BcMagTM Long-arm Carboxyl-terminated Magnetic Beads are recommended for conjugating small peptides because the long-arm hydrophilic linker may reduce steric hindrance.

The unique dry form eliminates the need for solvent storage or removal and disposal. Furthermore, because the dry resin concentrates the sample as it swells, lowering the volume of the starting material and resulting in highly effective ligand immobilization, it is perfect for coupling reactions with dilute materials.

Workflow

BcMag™ Carboxyl-Terminated Magnetic Beads work perfectly as affinity support for various bioseparations to refine molecules, cells, and parts into purified fractions. After conjugation with ligands, add the beads to a cell lysate containing the target molecules, then mix, incubate, wash and elute the target molecules.

Features and Advantages

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Easy to use

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Stable covalent bond with minimal ligand leakage

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Produces reusable immunoaffinity matrices

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Low nonspecific binding

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Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads

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Applications: Purification for antibodies, proteins/peptides, DNA/RNA, cell sorting, immunoprecipitation

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Instruction Manual

MSDS