BcMag™ Amine-terminated Magnetic Beads are magnetic beads coated with a high density of primary amine functional groups on the surface. The beads can covalently conjugate primary amine or carboxy-containing ligands such as protein and peptides for affinity purification. The hydrophilic surface ensures low nonspecific adsorption beads, excellent dispersion, and easy handling in various buffers. BcMag™ Amine-terminated Magnetic Beads are most suitable for conjugating a larger protein. BcMagTM Long-arm amine-terminated Magnetic Beads are recommended to conjugate small peptides because the long-arm (21-atom) hydrophilic linker may reduce steric hindrance.

The carboxyl group is found in a wide range of biological substances. Peptides and proteins contain carboxyl (-COOH) at the C-terminus of each polypeptide chain as well as aspartic acid (Asp, D) and glutamic acid side chains (Glu, E). Carboxyls, like primary amines, are typically found on the surface of protein structures.

Carboxylic acids can be employed to immobilize biological molecules by applying a carbodiimide-mediated process. Although no activated support has a reactive group that is spontaneously reactive with carboxylates, chromatographic supports containing amines (or hydrazides) can be utilized to create amide bonds with carboxylates activated using the water-soluble carbodiimide crosslinker EDC.

Workflow

BcMag™ Amine-Terminated magnetic beads work perfectly as solid resin for various affinity purifications to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a solution containing the target molecules, then mix, incubate, wash and elute the target molecules.

Features and Benefits

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Covalently coupled with high efficiency

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Easy to use

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Stable covalent bond with low levels of ligand leakage

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Produces reusable immunoaffinity matrices

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Low nonspecific binding

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Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads

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Application: Purification for antibody, protein/peptide, DNA/RNA; cell sorting, immunoprecipitation

Learn More

Instruction Manual

MSDS

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