The amine group (-NH2) is the most common functional target for immobilizing protein molecules: This group can be found at the N-terminus of each polypeptide chain (called the alpha-amine) and in the side chain of lysine (Lys, K) residues (called the epsilon-amine). Because of their positive charge under physiological conditions, primary amines are frequently outward facing (i.e., on the outside surface) of proteins; hence, they are usually available for conjugation without denaturing the protein structure.

BcMag™ NHS-Activated Magnetic Beads are uniform, magnetic beads coated with high-density NHS (N-hydroxyl succinimide) functional groups on the surface. The magnetic resin utilizes reliable NHS-ester chemistry and does not require the use of dangerous chemicals for immobilization. The beads have less nonspecific binding and no leaking of the coupled ligand. The resin can quickly, efficiently, and covalently conjugate any primary amine-containing ligands by forming stable amide linkages with better than 85% coupling efficiency. Bioclone NHS-Activated beads reactions take less than an hour (15–30 min at room temp pH 7–9, 4 hours at 4 °C) and produce substantially more stable linkages. BcMag™ Long-arm NHS-activated Magnetic Beads are recommended to immobilize small peptides because the long-arm hydrophilic linker may reduce steric hindrance. The magnetic resin can be employed in various affinity purification methods.

The unique dry form eliminates the need for acetone solvent storage or removal and disposal. Furthermore, because the dry resin concentrates the sample as it swells, lowering the volume of the starting material and resulting in highly effective ligand immobilization, it is perfect for coupling reactions with dilute materials.

Workflow

BcMag™ NHS-Activated Magnetic Beads work perfectly as solid support for a wide variety of affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a solution containing the target molecules, then mix, incubate, wash and elute the target molecules.

Features and Advantages

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Pre-activated and ready-to-use

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Easy to use

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A quick coupling in pH 7.4 at 4°C to 25°C

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Stable covalent bond with minimal ligand leakage

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Produces reusable immunoaffinity matrices

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Low nonspecific binding

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Immobilize 15-20 μg protein /mg beads

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Applications: Cell sorting, Immunoprecipitation; Purification for Antibodies, Proteins/Peptides, DNA/RNA

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Instruction Manual

MSDS