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当前位置: 首页 > 产品中心 > > Bioclone/1 μm BcMag™ NHS-Activated Magnetic Beads/150 mg/FI101
商品详细Bioclone/1 μm BcMag™ NHS-Activated Magnetic Beads/150 mg/FI101
Bioclone/1 μm BcMag™ NHS-Activated Magnetic Beads/150 mg/FI101
Bioclone/1 μm BcMag™ NHS-Activated Magnetic Beads/150 mg/FI101
商品编号: FI101
品牌: Bioclone Inc
市场价: ¥5500.00
美元价: 3300.00
产地: 美国(厂家直采)
公司:
产品分类: 其他
公司分类:
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

The amine group (-NH2) is the most common functional target for immobilizing protein molecules: This group can be found at the N-terminus of each polypeptide chain (called the alpha-amine) and in the side chain of lysine (Lys, K) residues (called the epsilon-amine). Because of their positive charge under physiological conditions, primary amines are frequently outward facing (i.e., on the outside surface) of proteins; hence, they are usually available for conjugation without denaturing the protein structure.

BcMag™ NHS-Activated Magnetic Beads are uniform, magnetic beads coated with high-density NHS (N-hydroxyl succinimide) functional groups on the surface. The magnetic resin utilizes reliable NHS-ester chemistry and does not require the use of dangerous chemicals for immobilization. The beads have less nonspecific binding and no leaking of the coupled ligand. The resin can quickly, efficiently, and covalently conjugate any primary amine-containing ligands by forming stable amide linkages with better than 85% coupling efficiency. Bioclone NHS-Activated beads reactions take less than an hour (15–30 min at room temp pH 7–9, 4 hours at 4 °C) and produce substantially more stable linkages. BcMag™ Long-arm NHS-activated Magnetic Beads are recommended to immobilize small peptides because the long-arm hydrophilic linker may reduce steric hindrance. The magnetic resin can be employed in various affinity purification methods.

NHS-activated support coupling reaction

The unique dry form eliminates the need for acetone solvent storage or removal and disposal. Furthermore, because the dry resin concentrates the sample as it swells, lowering the volume of the starting material and resulting in highly effective ligand immobilization, it is perfect for coupling reactions with dilute materials.

Workflow

BcMag™ NHS-Activated Magnetic Beads work perfectly as solid support for a wide variety of affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a solution containing the target molecules, then mix, incubate, wash and elute the target molecules.

Workflow of magnetic beads for affinity purification

Features and Advantages

Pre-activated and ready-to-use

Easy to use

A quick coupling in pH 7.4 at 4°C to 25°C

Stable covalent bond with minimal ligand leakage

Produces reusable immunoaffinity matrices

Low nonspecific binding

Immobilize 15-20 μg protein /mg beads

Applications: Cell sorting, Immunoprecipitation; Purification for Antibodies, Proteins/Peptides, DNA/RNA

Learn More

Instruction Manual

MSDS

Related Affinity Magnetic Beads  →
品牌介绍
Bioclone的用于学术研究和治疗应用的重组蛋白/ DNA的数量已大大增加。然而,成功的重组蛋白表达取决于许多因素,例如密码子偏好性,RNA二级结构,异源表达系统中的GC含量。越来越多的实验结果证明,与预优化相比,取决于不同的基因,表达水平显着提高,从两倍提高到一百倍。Bioclone开发了一个独特的专有技术平台,并生成了超过14,000个人工合成的,经过密码子优化的cDNA / DNA克隆(克隆在大肠杆菌表达载体中,图1)和重组蛋白(在大肠杆菌酵母中生产)。Bioclone为所有cDNA克隆和重组蛋白生产提供即用型和基于客户的服务。特别设计和合成了数十万种重组蛋白和密码子优化的cDNA (DNA开放阅读框)。  密码子优化的cDNA / DNA:    产生更高产量的重组蛋白。将cDNA / DNA 克隆克隆到具有6x His -tag的大肠杆菌表达载体中,可立即用于重组蛋白生产。可以使用作为验证的RNAi的功能由于在其〜30%差的RNAi的援助cDNA序列时相比原的cDNA / DNA 。Bioclone 还提供客户服务克隆中的cDNA插入NY 所需的客户向量小号。重组蛋白:重组蛋白C 在N末端或C末端具有6x His-tag重组蛋白P roduced在大肠杆菌或小号F9昆虫细胞。  provid 我纳克准备使用的重组蛋白和p rotein点播服务的所有cDNA克隆。通过SDS-PAGE 测定的重组蛋白纯度> 90%。ř ecombinant蛋白应用:Western印迹,ELISA 或可以用于其它应用。  cDNA克隆和重组蛋白包括:我nfection疾病抗原(病毒,细菌,寄生虫,细菌毒素),抗过敏的蛋白质,细胞因子,   激酶,磷酸酶,信号转导,   干细胞和发展,神经科学, 药物   metabollism,普通的病,转录因子,癌症和更重组蛋白质和克隆是g 还是机翼.......