Dandruff is a clinical alteration of the skin that consists histologically of orthokeratotic clumps with minute parakeratotic foci found in inflammatory pathologies such as seborrheic dermatitis and psoriasis. Therefore, some nucleated cells should be found in dandruff, and hence there is a possibility that forensically typeable DNA could be extracted. A previous study discovered that 1-1.5 mg of dandruff yielded 30-40 ng of DNA. However, the current DNA extraction technologies suffer from a tedious binding-washing-elution step, poor integrity, DNA losses, and toxic organic solvents. To overcome these drawbacks, we developed a novel, efficient one-step Dandruff DNA purification system.
BcMag™ One-Step Dandruff Cell DNA Purification Kit allows rapid and efficient purification of genomic DNA from Dandruff. It uses novel negative selection chromatography magnetic beads to quickly capture and remove the impurities, such as PCR inhibitors from cell lysate, leaving the DNA untouched. It reduces the risk of DNA loss and carryover of extraction buffers from the traditional tedious bind-wash-elute procedure. The purification kit provides a fast and straightforward DNA extraction method with only one tube, no liquid transfer, and no requirement for carrier RNA. After preparing the lysates, Hundreds of samples can be processed in less than 30 minutes without using expensive equipment.
Principle and Workflow
The specially designed magnetic beads with our proprietary surface chemistry function capture the impurity once mixed with the sample. The magnetic beads-impurity complex is then magnetically removed by a magnet while the pure DNA remains in the solution.
1.
Add functional magnetic beads to the sample.
2.
Mix the samples with the magnetic beads and proteinase K to lyse the cells.
3.
Mix by vertexing/pipetting for the beads to capture the PCR inhibitors.
4.
Remove the beads with a magnet.
5.
Aspirate the supernatant containing the pure ready-to-use DNA/RNA.
Performance
The purified DNA is suitable for use in sensitive downstream applications, such as PCR, qPCR, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, genotyping, or next-generation sequencing (NGS), buccal DNA sample collection, veterinary genotyping and diagnostics, research genotyping, Veterinary genotyping and diagnostics, genetic testing, forensics, population studies.
Features and Advantages
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Rapid and efficient purification protocol: without prior DNA isolation for subsequent use in direct workflows, No liquid transfer, and One-tube.
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Ultrafast: Process 96 samples in less than an hour.
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Highest nucleic acids recovery rates: Minimal loss of DNA during extraction.
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Effectively removes inhibitors: polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, denim dyes, divalent cations such as Ca2+, Mg2+, etc.●
Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and organic reagents.
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High throughput: Compatible with many different automated liquid handling systems.
Learn More
Instruction Manual
MSDS
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