Fingerprint or touch DNA is obtained from shed skin cells and other biological material left behind on surfaces during physical contact. This type of DNA evidence can be useful in forensic investigations, but fingerprint samples can present several challenges. One of the main problems is that only a relatively low percentage of fingerprints have been successfully amplified and typed using traditional DNA extraction methods. These methods often involve a multi-step process that is time-consuming, can lead to poor integrity of the DNA, and can result in significant DNA loss. Additionally, traditional methods can also require the use of toxic organic solvents. To overcome these challenges, Bioclone has developed new one-step fingerprint DNA purification systems, such as the BcMag™ One-Step Fingerprint DNA Purification Kit, which utilizes negative selection chromatography magnetic beads to quickly capture and remove impurities from cell lysate, leaving the DNA untouched. This method is designed to be fast, efficient, and easy to use, with minimal risk of DNA loss or carryover of extraction buffers. It only requires one tube, no liquid transfer, and no carrier RNA, allowing for rapid processing of hundreds of samples in less than 30 minutes without the need for expensive equipment. This one-step purification system is considered revolutionary in solving the problems associated with traditional fingerprint DNA purification methods.

Principle, Workflow, and Results

The principle of the BcMag™ One-Step Fingerprint DNA Purification Kit is based on negative selection chromatography using magnetic beads. The kit utilizes specially designed magnetic beads with a proprietary surface chemistry that can capture impurities, such as PCR inhibitors, from the sample. Once mixed with the sample, the magnetic beads bind to the impurities and form a magnetic beads-impurity complex. The workflow of the purification process involves three main steps: Sample Preparation: The biological sample is lysed and processed to release the DNA. Purification: The lysate is mixed with the magnetic beads, and the impurities are captured by the beads. The magnetic beads-impurity complex is then magnetically removed from the solution by using a magnet. Elution: The pure DNA remains in the solution and can be easily transferred by simply pipetting the DNA-containing solution to a new tube. Overall, the BcMag™ One-Step Fingerprint DNA Purification Kit provides a fast and straightforward DNA extraction method with only one tube, no liquid transfer, and no requirement for carrier RNA, allowing for rapid processing of hundreds of samples in less than 30 minutes without the need for expensive equipment.

Performance

BcMag™ One-Step Fingerprint DNA Purification Kit is designed to provide high-quality DNA that is suitable for a wide range of downstream applications. The purified DNA is free of contaminants and PCR inhibitors, which can interfere with downstream reactions and reduce the sensitivity of PCR-based assays. Some of the features and advantages of this kit include:

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Rapid and efficient purification protocol: The one-step protocol eliminates the need for multiple washing and elution steps, and the use of carrier RNA is not required.

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High-throughput: The kit allows for the processing of 96 samples in less than an hour, making it suitable for high-throughput applications.

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High nucleic acids recovery rates: The kit is designed to minimize DNA loss during extraction.

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Cost-effective: The kit eliminates the need for columns, filters, laborious repeat pipetting, and organic reagents, making it a cost-effective option.

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Compatible with automated liquid handling systems: The kit is compatible with many different automated liquids handling systems, making it a suitable option for high-throughput applications.

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Overall, the BcMag™ One-Step Fingerprint DNA Purification Kit offers a fast, efficient, and cost-effective method for purifying DNA from fingerprint or touch DNA samples.

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Instruction Manual

MSDS