The process of extracting DNA from mouse tails or ears can be very time-consuming and requires a lot of labor. Specific applications, such as genotyping transgenic mice using a tail section, demand a lengthy DNA extraction method. Traditional methods involve a long process of digesting the tissue with proteinase K for several hours, followed by using chemicals like phenol and chloroform to extract the DNA and then precipitating it with alcohol. Recently, new techniques have emerged that use silica-based kits to purify DNA. These kits use high concentrations of a salt called guanidine hydrochloride to bind the DNA to silica. After binding, the DNA is washed with a solution of salt and ethanol to remove other substances from the sample, and then eluted with a buffer or water to obtain pure DNA. However, these methods can also be time-consuming and require multiple washing and spinning steps, which can result in DNA loss and shearing. Additionally, impurities can easily pass through the eluted DNA, affecting its purity and quantification, and potentially affecting downstream processes like PCR.
BcMag™ One-Step Mouse Tail DNA Purification Kit allows rapid, column-free extraction of genomic DNA from mouse tail, ear, ear punch, or other animal tissue. The kit uses our unique proprietary magnetic beads and buffers to efficiently lyse cells and remove all impurities simultaneously in an aqueous buffer, leaving the DNA untouched. The procedure employs mild lysis conditions, avoiding harsh conditions such as alkaline lysis and toxic chemicals for lysing cells to maintain DNA integrity and the time-consuming cleanup of organic solvent from the sample. Furthermore, the magnetic beads eliminate PCR inhibitors (Fig.1) from samples in a single step without DNA extraction.
It increases DNA integrity, boosts nucleic acid yields, and minimizes DNA loss caused by typical DNA extraction techniques’ time-consuming “bind-wash-elute” procedure. Following sample lysis, the straightforward one-step extraction technique enables simultaneous processing of >96 samples and produces pure DNA in less than 30 minutes. Purified genomic DNA has the highest integrity and can be used in various downstream applications such as qPCR.
Workflow of one-step mouse tail DNA isolation kit
The specially designed magnetic beads can quickly capture the impurity once mixed with the cell lysate. Then, the magnetic beads-impurity complex is magnetically removed by a magnet while the pure DNA remains in the solution.
1.
Add magnetic beads to the sample.
2.
Mix the samples with the magnetic beads and proteinase K and heat to lyse the cells.
3.
Mix by vortexing/pipetting for the beads to capture the PCR inhibitors.
4.
Remove the beads with a magnet.
5.
Aspirate the supernatant containing the pure ready-to-use DNA.
Performance
The purified DNA is ready for downstream applications, such as PCR, qPCR, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, genotyping, next-generation sequencing (NGS, veterinary genotyping, etc.
Features and Advantages
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Rapid and efficient extraction protocol: without prior DNA isolation for subsequent use in direct workflows, No liquid transfer, and One-tube.
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Ultrafast: Process 96 samples in less than an hour.
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Highest nucleic acids recovery rates: Minimal loss of DNA during extraction.
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Effectively cell lysate cleanup and removes inhibitors: polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, denim dyes, divalent cations such as Ca2+, Mg2+, etc.●
Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and organic reagents.
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High-throughput: Compatible with many different automated liquid handling systems.
Learn More
Instruction Manual
MSDS
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