The accurate and rapid diagnosis of viral diseases is critical for early therapeutic intervention and effective treatment. Nucleic acid detections are among the most sensitive and specific tools for viral diagnosis, while the high quality of the purified nucleic acids is crucial for successful detection. Due to the very low amount of virus present in the samples, the nucleic acid purification techniques must be a robust and reliable extraction system to deliver pure and high-quality nucleic acids for sensitive downstream applications. Currently available viral Nucleic acid purification kits present challenges such as low DNA yield, time-consuming, laborious, or contamination with PCR inhibitors. They require expensive laboratory settings and well-trained personnel. To overcome these hurdles, Bioclone offers a novel extraction-free nucleic acid purification technique for trace amounts of viral samples, a robust alternative for viral nucleic acid extraction that guarantees a stable and scalable high-throughput extraction workflow at a low cost from the sample.
BcMag™ One-Step Saliva Viral RNA-DNA Purification Kit uses novel negative selection chromatography magnetic beads to quickly capture impurities such as PCR inhibitors from cell lysate, leaving the DNA /RNA untouched. Unlike standard tedious bind-wash-elute protocol, this convenient single-step and the extraction-free procedure does not contain traces of organic solvents, chaotropic salts, or EDTA, reducing the risk of DNA/RNA loss and carryover of extraction buffers, and recovers almost 100% DNA/RNA. The purification kit provides a fast and simple method for DNA/RNA purification with only one tube, no liquid transfer, and no requirement for carrier RNA. Hundreds of trace samples such as saliva and nasal-pharyngeal swabs in saline solution, nasal swabs, buccal swabs & sputum/saliva swabs can be processed in less than 15 minutes without using expensive types of equipment. The purified nucleic acid is ready for downstream applications: PCR, RT-PCR, RT-qPCR, LAMP, RT-LAMP.
Workflow
1.
Add functional magnetic beads to the sample.
2.
Mix the samples with the magnetic beads and heat to lyse the cells.
3.
Mix by pipetting or vortexing to capture the impurity.
4.
Magnetic capture the beads and aspirate the supernatant containing the pure ready-to-use DNA/RNA
Performance
The purified DNA is suitable for use in sensitive downstream applications, such as PCR, qPCR, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, genotyping, or next-generation sequencing (NGS), buccal DNA sample collection, veterinary genotyping and diagnostics, research genotyping, Veterinary genotyping and diagnostics, genetic testing, forensics, population studies.
Features and Advantages
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Rapid and efficient purification protocol: without prior DNA isolation for subsequent use in direct workflows, No liquid transfer, and One-tube.
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Ultrafast: Process 96 samples in less than an hour.
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Highest nucleic acids recovery rates: Minimal loss of DNA during extraction
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Effectively removes inhibitors: polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, denim dyes, divalent cations such as Ca2+, Mg2+, etc.●
Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and organic reagents.
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High throughput: Compatible with many different automated liquid handling systems.
Learn More
Instruction Manual
MSDS
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