Fungi and yeast are essential microorganisms in many research fields, including medical, agricultural, and environmental sciences. However, their DNA extraction can be a challenging and time-consuming process.

Standard procedures for extracting fungi and yeast DNA can be exceedingly Time consuming and labor intensive. Specific applications, such as fungi, and yeast genotyping, demand a lengthy DNA extraction method. For example, traditional fungi and yeast DNA extractions need a 4-hour to overnight digestion with proteinase K, followed by phenol: chloroform extraction and alcohol precipitation. Other techniques, such as silica bind and elute kits, have recently become available. Those techniques are based on positive chromatography purifying selection. High chaotropic salt concentrations, such as guanidine hydrochloride, bind DNA to silica. The silica column or beads is washed with a salt/ethanol solution after nucleic acid binding to eliminate additional biomolecules from the sample. Finally, the column or beads is eluted using Tris elution buffer or water to remove the pure DNA. Such bind-wash-elute procedures are time-consuming, requiring multiple washing and spinning steps. Repetitive spin steps can cause considerable DNA loss (20-40%) and shearing. Furthermore, chaotropic salts and other impurities can easily pass through the eluted DNA or RNA, compromising ultimate purity and quantification as well as downstream enzymatic activities like PCR.

Bioclone has developed a kit called ” One-Step Fungi and Yeast DNA Purification kit ” to tackle the challenges associated with extracting DNA from fungi and yeast.

The One-Step Fungi & Yeast DNA Purification Kit is an essential tool for efficient and rapid DNA extraction from microorganisms such as fungi and yeast. Its unique proprietary magnetic bead and buffer system offers gentle cell lysis and simultaneous impurity removal, avoiding harsh conditions and toxic chemicals. The one-step purification technique allows the simultaneous processing of many samples, increasing DNA integrity and reducing DNA loss. The purified genomic DNA is of the highest quality and suitable for various downstream applications such as qPCR. With this kit, researchers can streamline their DNA extraction process, saving time and reducing cost.

Workflow of one-step fungi, yeast DNA Purification kit

1.

Use bead beating to disrupt the sample in a bead beater.

2.

Centrifuge and transfer the supernatant to a new tube.

3.

Mix the samples with the magnetic beads and proteinase K and heat to lyse the cells.

4.

Vortex the beads to capture PCR inhibitors.

5.

Remove the beads with a magnet.

6.

Aspirate supernatant containing the pure ready-to-use DNA.

Proprietary Magnetic Beads and Buffers for Efficient Cell Lysis and Impurity Removal

The One-Step Fungi & Yeast DNA Purification Kit employs a unique proprietary magnetic bead and buffer system to efficiently lyse cells and remove PCR inhibitor (polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, denim dyes, divalent cations such as Ca2+, Mg2+, etc.) simultaneously in an aqueous buffer, leaving the DNA untouched. This gentle lysis method maintains DNA integrity and avoids harsh conditions such as alkaline lysis and toxic chemicals. Moreover, the magnetic beads eliminate PCR inhibitors from the samples in a single step, enhancing DNA integrity, boosting nucleic acid yields, and minimizing DNA loss.

One-Step Purification Technique for High-Quality DNA

The One-Step Fungi & Yeast DNA Purification Kit features a one-step purification technique that enables the simultaneous processing of more than 96 samples and produces pure DNA in less than 30 minutes. This straightforward approach eliminates the time-consuming “bind-wash-elute” procedure of typical DNA extraction techniques and increases DNA integrity. The purified genomic DNA has the highest quality and is suitable for various downstream applications such as qPCR.

Advantages of One-Step Fungi & Yeast DNA Purification Kit

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Rapid and efficient DNA extraction from fungi and yeast: One-step, one tube, no liquid transfer.

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Unique proprietary magnetic bead and buffer system for gentle cell lysis and impurity removal

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Single-step PCR inhibitor elimination

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High-quality DNA suitable for downstream applications

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Simultaneous processing of >96 samples

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No column required, reducing extraction time and cost.

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Instruction Manual

MSDS