A sulfhydryl is a functional group that contains a sulfur atom linked to a hydrogen atom. In chemistry, the sulfhydryl group, often known as a thiol, is denoted by the suffix “-thiol” and the prefix “mercapto-” or “sulfanyl.” Thiols have a strong preference for soft metals.

BcMag™ Sulfhydryl-Terminated Magnetic Beads are uniform, silica-based superparamagnetic beads grafted with a high density of sulfhydryl (SH) functional groups on the surface. The Sulfhydryl-Magnetic Beads are specially designed for conjugation of protein/peptides modified by SMCC (Sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate). SMCC contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The procedure is quick and straightforward for sample preparation without laborious repeat pipetting and centrifuging. BcMagTM Sulfhydryl-Terminated Magnetic Beads are ideal for conjugating large proteins. BcMagTM Long-Arm Sulfhydryl-Terminated Magnetic Beads are recommended for conjugating small peptides.

Workflow

BcMag™ Sulfhydryl-Terminated Magnetic Beads work perfectly as solid resin for various affinity purifications to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a solution containing the target molecules, then mix, incubate, wash and elute the target molecules.

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Instruction Manual

MSDS